virotype BTV pan/8 RT-PCR Kit

(1 customer review)

Liên hệ

Kit phát hiện 27 serotypes đã biết của bluetongue virus (BTV) và phân biệt European BTV serotype 8 (BTV-8)

Hãng sản xuất: Qiagen - Đức

Separate detection of the European BTV serotype 8 (BTV-8)
Easy-to-use single reaction mix containing controls
RT-PCR run time of less than 2 hours
Can be performed in one cycler run with other virotype RT-PCR assays

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1. Thông tin kit

The virotype BTV pan/8 RT-PCR Kit allows the safe detection of RNA from bluetongue virus (BTV) in samples from cattle, sheep, and goats. The kit contains all of the reagents for multiplex real-time RT-PCR in a single, ready-to-use reaction mix. It can be used with pools of up to 10 individual samples of blood and with tissue samples from the spleen and lymph nodes.

A validation study of 338 blood samples, with known BTV status (174 positive and 164 negative; kindly provided by state laboratories), was tested with the virotype BTV pan/8 RT-PCR Kit. In this study, all positive samples tested positive and all negative samples tested negative, providing a diagnostic sensitivity and specificity of 100%. In addition, a titration series was performed with ten-fold dilutions from 10⁷ to 1 copy/well of BTV RNA. The results demonstrate a high correlation (99%) between the number of copies and the amount of amplification product in the range from 10⁷ to 100 copies/well, and positive detection of the virus down to 1 copy/well

2. Thông tin đặt hàng

Cat No./ID: 280443 virotype BTV pan/8 RT-PCR Kit (24)

For 24 reactions: Master Mix, Positive Control, Negative Control

Cat No./ID: 280445 virotype BTV pan/8 RT-PCR Kit (96)

For 96 reactions: Master Mix, Positive Control, Negative Control

Cat No./ID: 280453 virotype BTV pan/4 RT-PCR Kit (24)

For 24 reactions: Master Mix, Positive Control, Negative Control

Cat No./ID: 280455virotype BTV pan/4 RT-PCR Kit (96)

For 96 reactions: Master Mix, Positive Control, Negative Control